| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.117932 |
| Chromosome: | scaffold_24 |
| Location: | 120736 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre24.g755997 | PHC18,FAP150 | Cell wall protein pherophorin-C18 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTAGGTCAGCGAGATACACATAACGCATTC |
| Internal bar code: | GTGCGGTATCGTTCCTTGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.6689 |
| LEAP-Seq n confirming: | 1505 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGGTACACCTTCATCAC |
| Suggested primer 2: | GGATGTGGATTAGGGTGTGG |