Insertion junction: LMJ.RY0402.117957_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.117957
Chromosome:chromosome 3
Location:7417911
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATCCCTTTCGATCCCGAAGTTGGTCACT
Internal bar code:GCGGCGAGGGCAGGTCGCTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:237
LEAP-Seq percent confirming:99.7782
LEAP-Seq n confirming:17098
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCCTGCTCCTCGTTTGAC
Suggested primer 2:CCAGTGAAACCTTCCACGAT