Insertion junction: LMJ.RY0402.117974_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.117974
Chromosome:chromosome 10
Location:611007
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g421900 SRR27 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGATCGTTCCAGGGGCGTTCGATGCTGG
Internal bar code:CCTTACATAGGGTTATAGTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:890
LEAP-Seq percent confirming:99.3711
LEAP-Seq n confirming:316
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTCCGTGTGTGCATGTTT
Suggested primer 2:AATCACGGTGCATCAAACAA