Insertion junction: LMJ.RY0402.117986_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.117986
Chromosome:chromosome 6
Location:5360244
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g283550 CRC2,POC6 Proteome of centriole protein 6; (1 of 1) K16466 - centrin-3 (CETN3, CDC31) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAGCGTTACAGAGTGCACAGATGTCAGA
Internal bar code:ACGCGGGGTTTTTGCTTGAACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:879
LEAP-Seq percent confirming:99.88
LEAP-Seq n confirming:832
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AACTGGACTTCCAGGTGTGG
Suggested primer 2:GCGACGCTGAAGACATATCA