| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.117995 |
| Chromosome: | chromosome 10 |
| Location: | 3219679 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g442750 | (1 of 1) K19045 - E3 ubiquitin-protein ligase BIG BROTHER and related proteins [EC:6.3.2.19] (BB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTCGGCCCTTCGCGGCATACCCGCCGC |
| Internal bar code: | TTATTGCCGATAGCAATCTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 99.5458 |
| LEAP-Seq n confirming: | 1315 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTACCACCCTCTTGCCAT |
| Suggested primer 2: | AACGCCTGAGGACTTCTTGA |