Insertion junction: LMJ.RY0402.118004_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.118004
Chromosome:chromosome 2
Location:7172830
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g146400 CSB1,CSB2 Predicted protein of CSB family 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCACGCGCACGTGCCTGACACAAAGTCC
Internal bar code:TATAATGGTTTCTACGTCCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGTCCCTTCTTGCTACGC
Suggested primer 2:CAGCTAAGCCTTCAACGGTC