| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118036 |
| Chromosome: | chromosome_5 |
| Location: | 3208857 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g239850 | PHO4,PHOD | Alkaline phosphatase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGACGCTCCTCTCGTCGTGACGCCCCTGAC |
| Internal bar code: | CCCAGCCGCGGAGAGGAAACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 99.5258 |
| LEAP-Seq n confirming: | 2099 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTACCACCCTCCTCCTTC |
| Suggested primer 2: | GGGGTGTGTGTGTGTTTCTG |