| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118054 |
| Chromosome: | chromosome 16 |
| Location: | 3680020 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688700 | (1 of 22) PF01764 - Lipase (class 3) (Lipase_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAATATGAAGTCTCGACGTCTTGGAAGG |
| Internal bar code: | GCCACCGAGTTAAGGTAAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 99.6431 |
| LEAP-Seq n confirming: | 3071 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCTGATGGCTCCGTCTTG |
| Suggested primer 2: | CCAGCAATCGAGTCTCATCA |