| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118109 |
| Chromosome: | chromosome 12 |
| Location: | 7838883 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553100 | (1 of 1) PF16529 - WD40 region of Ge1, enhancer of mRNA-decapping protein (Ge1_WD40) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACTTGTCAGCGTCCAATCCATGCGCAT |
| Internal bar code: | AGGAACGTGTCCTCCTGGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 99.3382 |
| LEAP-Seq n confirming: | 1351 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACACTGAGGCGGTCTTG |
| Suggested primer 2: | GAGGAGGCTGAAGTGGTGAG |