| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.118125 |
| Chromosome: | chromosome 1 |
| Location: | 7305291 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052650 | APT2,VIP2 | inositol hexakisphosphate kinase; (1 of 2) 2.7.4.21 - Inositol-hexakisphosphate kinase / IP6K | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACTACACACACCGCGCACACACAATGA |
| Internal bar code: | GGGTACTAGATGCGCCGCATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTCCAGCATCAGCCTGT |
| Suggested primer 2: | AAGCAAGCTAGGACACGCAT |