Insertion junction: LMJ.RY0402.118182_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.118182
Chromosome:chromosome 14
Location:1501226
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g618400 ABCA3 (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase; ABC transporter 3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCTCCTTACTACGGCCTTGCGGTACCA
Internal bar code:TTACTTCAGTATACCTGATTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:185
LEAP-Seq percent confirming:99.5676
LEAP-Seq n confirming:1842
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTGCGACATGTAAGTGG
Suggested primer 2:CGAGTCCTCGAAGAAGGATG