| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118198 |
| Chromosome: | chromosome 6 |
| Location: | 1688380 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261200 | ERG5,ERG25 | Sterol desaturase/putative lathosterol oxidase; (1 of 1) PTHR11863//PTHR11863:SF2 - STEROL DESATURASE // PROTEIN ECERIFERUM 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCAGTGTTATCGGCTTATCACTCCAGCA |
| Internal bar code: | ACGGTAAAGAAGGGACTTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 334 |
| LEAP-Seq percent confirming: | 98.5646 |
| LEAP-Seq n confirming: | 206 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACTACTTCCCGACCAAGG |
| Suggested primer 2: | GCTGACACCCCTTGTCGTAT |