| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118215 |
| Chromosome: | chromosome_2 |
| Location: | 6292023 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g114600 | PRX2 | 2-cys peroxiredoxin | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATGGGCCCGACCCCCCACACGTCTCGCAG |
| Internal bar code: | AGGATATCGCGGGAAGAGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 1674 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGATACCGCGTGTTGAACC |
| Suggested primer 2: | ATACGCATTTTTGGGCTGTC |