| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118232 |
| Chromosome: | chromosome 7 |
| Location: | 58531 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312400 | KDG1 | Diacylglycerol kinase; (1 of 2) K00901 - diacylglycerol kinase (ATP) (dgkA, DGK) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACTCAGGCCCGGGTTTTAGTGTTCACA |
| Internal bar code: | CTACAGCCTGGGTGCGTGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 99.2678 |
| LEAP-Seq n confirming: | 12609 |
| LEAP-Seq n nonconfirming: | 93 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGCGTAGTTACCCGATT |
| Suggested primer 2: | TTGCCCAATTCTGTGTGTGT |