Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.118241 |
Chromosome: | chromosome_6 |
Location: | 6509642 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g293150 | PGM12 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCCAAAGATGCTCTGCACCCCTGCATGCT |
Internal bar code: | GTTGCTGACCACCAGCAATATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 99 |
LEAP-Seq percent confirming: | 98.4375 |
LEAP-Seq n confirming: | 63 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTGCGCGGGAATATAACAG |
Suggested primer 2: | CTTCTCTTCCCTCCCTGTCC |