| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.118251 |
| Chromosome: | chromosome 17 |
| Location: | 1656887 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708250 | ARL8 | (1 of 1) K07955 - ADP-ribosylation factor-like protein 8 (ARL8); ARF-like GTPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTGGCAGTGGATGGAGCCTGTCCTACA |
| Internal bar code: | CAGTTCGCGTCGAAAGGTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 407 |
| LEAP-Seq percent confirming: | 98.9247 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAGTGCGGAAAATGACC |
| Suggested primer 2: | AAATCCACCCTCAACTGTGC |