| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118273 |
| Chromosome: | chromosome 16 |
| Location: | 519939 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692800 | AKR4 | Aldo/keto reductase; (1 of 1) PTHR11732//PTHR11732:SF284 - ALDO/KETO REDUCTASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGTAAAGCTCTACTGCGGGCGCGCCGGG |
| Internal bar code: | GTCGCTGGTATGAAACTGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 99.7815 |
| LEAP-Seq n confirming: | 6393 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACCCTTGCTTACCCT |
| Suggested primer 2: | TGTTTGTGTGGGCTTGTCAT |