Insertion junction: LMJ.RY0402.118291_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.118291
Chromosome:chromosome 9
Location:73940
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g386250 SMM33 S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAACGCCGGTGGAGGTGTGTGCCACTTT
Internal bar code:TACTCTTTTTTTAGTCATAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:936
LEAP-Seq percent confirming:99.8287
LEAP-Seq n confirming:1748
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCCACAATACACCGTCCT
Suggested primer 2:ACAGCGCACACACTAACCAG