Insertion junction: LMJ.RY0402.118291_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.118291
Chromosome:chromosome 9
Location:74024
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g386250 SMM33 S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGTAGCCGCATGGTCATCTCCAGCCGCA
Internal bar code:TTGGCGCACACCGTAGACGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:660
LEAP-Seq percent confirming:99.8403
LEAP-Seq n confirming:4377
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCCACAATACACCGTCCT
Suggested primer 2:GCGCCTTCCAATATCACAGT