| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118295 |
| Chromosome: | chromosome 6 |
| Location: | 5182505 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCTGCGGGGGCATCGTGTGGGCGGGGAG |
| Internal bar code: | GTTCGCTAGTTGGCAGGAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 94.3942 |
| LEAP-Seq n confirming: | 1044 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAACAAAGCAGTCCAAA |
| Suggested primer 2: | AAAAACTGCTGCCATGCTTT |