| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118321 |
| Chromosome: | chromosome 16 |
| Location: | 7134497 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680678 | TRZ2 | RNase Z-like protein; (1 of 2) PTHR12553//PTHR12553:SF49 - RIBONUCLEASE Z // ZINC PHOSPHODIESTERASE ELAC PROTEIN 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGTGGTGTCGGGCCTGGCGTCCTTCGTG |
| Internal bar code: | ACGTTAGGGGGCGGGTGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 75.8644 |
| LEAP-Seq n confirming: | 65650 |
| LEAP-Seq n nonconfirming: | 20886 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTATTTCAGCACCCAGCG |
| Suggested primer 2: | ATAGTTAGTGGGCGGGCTTT |