| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.118327 |
| Chromosome: | chromosome 10 |
| Location: | 1662234 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429800 | COQ8,ABC1 | Ubiquinone biosynthesis protein; (1 of 1) PTHR10566:SF65 - UBIQUINONE BIOSYNTHESIS PROTEIN COQ-8 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAAGCGCCCTCACAGCCGTGGCGCTCC |
| Internal bar code: | TACGGAAAAAACTAGGACTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 99.6756 |
| LEAP-Seq n confirming: | 6145 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGGTCCTAAAGGGAGGG |
| Suggested primer 2: | CTGCTTGAGCTAGGACCGAC |