Insertion junction: LMJ.RY0402.118329_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.118329
Chromosome:chromosome 16
Location:2818092
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g663200 FAP295 (1 of 2) PTHR24353//PTHR24353:SF37 - CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE // PROTEIN KINASE DC1-RELATED; Flagellar Associated Protein 295 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTTGTGGTTCTTGATGTCCATGGCGCGC
Internal bar code:GCGGTTGTCCAAACTTTGCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:922
LEAP-Seq percent confirming:94.0778
LEAP-Seq n confirming:2081
LEAP-Seq n nonconfirming:131
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CATAAGCACCTGTCTTGCGA
Suggested primer 2:TTGCATGTCCCGTAATCGTA