| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.118329 |
| Chromosome: | chromosome_16 |
| Location: | 2818092 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g663200 | FAP295 | Flagellar Associated Protein, cyclic nucleotide dependent protei | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATTTGTGGTTCTTGATGTCCATGGCGCGC |
| Internal bar code: | GCGGTTGTCCAAACTTTGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 922 |
| LEAP-Seq percent confirming: | 94.0778 |
| LEAP-Seq n confirming: | 2081 |
| LEAP-Seq n nonconfirming: | 131 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAAGCACCTGTCTTGCGA |
| Suggested primer 2: | TTGCATGTCCCGTAATCGTA |