Insertion junction: LMJ.RY0402.118332_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.118332
Chromosome:chromosome 3
Location:2041384
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g156300 FAP405 (1 of 1) PTHR28656:SF1 - COILED-COIL DOMAIN-CONTAINING PROTEIN 153; Flagellar Associated coiled-coil protein 405 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAAGTTGACTGGCATGCAGATACATAGG
Internal bar code:AACTATAATCAATCATCATCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:562
LEAP-Seq percent confirming:98.8493
LEAP-Seq n confirming:2749
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CATTCGGCATCTGCTACTGA
Suggested primer 2:GCAAACCAAAGAATCAGGGA