| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.118332 |
| Chromosome: | chromosome 3 |
| Location: | 2041384 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156300 | FAP405 | (1 of 1) PTHR28656:SF1 - COILED-COIL DOMAIN-CONTAINING PROTEIN 153; Flagellar Associated coiled-coil protein 405 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAAGTTGACTGGCATGCAGATACATAGG |
| Internal bar code: | AGGCAATTGAGAGGGCCGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 344 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCGGCATCTGCTACTGA |
| Suggested primer 2: | GCAAACCAAAGAATCAGGGA |