| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118332 |
| Chromosome: | chromosome 10 |
| Location: | 1258085 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426800 | (1 of 1) IPR000104//IPR003480 - Antifreeze protein, type I // Transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGACTTCACTAAATTCATGAAAGCGGGA |
| Internal bar code: | CCGACGGCTCGCATCGTGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 905 |
| LEAP-Seq percent confirming: | 98.2468 |
| LEAP-Seq n confirming: | 33455 |
| LEAP-Seq n nonconfirming: | 597 |
| LEAP-Seq n unique pos: | 181 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCGTCAAGGAGCAGAC |
| Suggested primer 2: | GTCAAGGCCTCTTCAAGTGC |