| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118351 |
| Chromosome: | chromosome 6 |
| Location: | 3958820 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278191 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAGCTCCAGAGGATGGCTGGGACCGAC |
| Internal bar code: | ACGGGAGCAACGGTGCTAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 98.6855 |
| LEAP-Seq n confirming: | 16892 |
| LEAP-Seq n nonconfirming: | 225 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTCCTCATCATGGAAGT |
| Suggested primer 2: | GGAAAGCGGATCACATGTTT |