| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.118363 |
| Chromosome: | chromosome 2 |
| Location: | 6834660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119300 | (1 of 1) PF14368 - Probable lipid transfer (LTP_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAGAGCTCCCGAGCGGGCGTCCAAGGG |
| Internal bar code: | AGAGCCTTTAGCGGATGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 280 |
| LEAP-Seq percent confirming: | 99.8716 |
| LEAP-Seq n confirming: | 1555 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTTGCACGAGTTCTGGA |
| Suggested primer 2: | AATGTGAGTCGTGGTGTGGA |