Insertion junction: LMJ.RY0402.118382_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.118382
Chromosome:chromosome 1
Location:1508006
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g007737 FBT1 Folate/pteridine transporter 1; (1 of 1) IPR004324//IPR011701//IPR020846 - Folate-biopterin transporter // Major facilitator superfamily // Major facilitator superfamily domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATCTTGAGATGTCAGCCACTCGAATCGC
Internal bar code:GCGGATTCCACCTTAGACTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:585
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:339
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGTCCTGCTAGCTTATTG
Suggested primer 2:GCGTAGCAAAGCTGGATAGG