Insertion junction: LMJ.RY0402.118385_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.118385
Chromosome:chromosome 17
Location:1515276
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g707150 LIC2 Hypothetical protein; (1 of 45) IPR029052 - Metallo-dependent phosphatase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGCTGCACTCACTGCCGCCCCTGCTTCT
Internal bar code:CCTACCTCCCTAATAAGAGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:599
LEAP-Seq percent confirming:61.5785
LEAP-Seq n confirming:1537
LEAP-Seq n nonconfirming:959
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCCTGCTACGCTATCCAC
Suggested primer 2:TACCTCTCACCACCCTCCAC