| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.118399 |
| Chromosome: | chromosome 5 |
| Location: | 362369 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233305 | (1 of 1) K07071 - uncharacterized protein (K07071) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCAAGTGATAGGCCCTGTGGGGCCGGTG |
| Internal bar code: | CGGATTGAACCCGCGGTCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 99.5208 |
| LEAP-Seq n confirming: | 623 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGACTAAACCGAACCCAA |
| Suggested primer 2: | AACCGTTACAGGATAGGGGG |