| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118417 |
| Chromosome: | chromosome 16 |
| Location: | 7064264 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678997 | (1 of 2) 2.4.2.38 - Glycoprotein 2-beta-D-xylosyltransferase / Beta-1,2-xylosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAGCAGAGCCCCTTTCCTCCGAGTTGG |
| Internal bar code: | ATGTCCAGGGCCCGGTCGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 717 |
| LEAP-Seq percent confirming: | 99.8066 |
| LEAP-Seq n confirming: | 5676 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAACTGTGCTCGTCCTCC |
| Suggested primer 2: | ACACTGCCTTGAGAGGGAGA |