| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.118452 |
| Chromosome: | chromosome 2 |
| Location: | 1899757 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087700 | APX1 | (1 of 1) PTHR31356//PTHR31356:SF1 - FAMILY NOT NAMED // L-ASCORBATE PEROXIDASE S, CHLOROPLASTIC/MITOCHONDRIAL-RELATED; Ascorbate peroxidase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACGTGGAGCGCCATGCCAGGCCGTTGC |
| Internal bar code: | TAAAATAAACTTTTCCGCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1242 |
| LEAP-Seq percent confirming: | 99.568 |
| LEAP-Seq n confirming: | 461 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAGTTGTCACCTTGAGT |
| Suggested primer 2: | TTATGTTACATGCCCGCTCA |