Insertion junction: LMJ.RY0402.118513_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.118513
Chromosome:chromosome 11
Location:3791873
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g483351 PHC44 Putative pherophorin-chlamydomonas homolog; (1 of 1) IPR003072//IPR003882//IPR024616 - Orphan nuclear receptor, NOR1 type // Pistil-specific extensin-like protein // Pherophorin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGGGGGGGGCTGGGAGGCGCCGGGCTA
Internal bar code:CCGCGGAAGCCATGCGCGAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:680
LEAP-Seq percent confirming:97.5
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGTTGGGAGCCAGACAT
Suggested primer 2:CTCGCCTATGCCTCCATCT