| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118530 |
| Chromosome: | chromosome 2 |
| Location: | 6843153 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGTGTGTGCAGGGGCTCCGGCGTGCT |
| Internal bar code: | CAGAAGAGAACCGCGAAGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 192 |
| LEAP-Seq percent confirming: | 85.6209 |
| LEAP-Seq n confirming: | 262 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGCGTGTGTGTGTGT |
| Suggested primer 2: | GTACTGACCGCCTGGTTCAT |