| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.118546 |
| Chromosome: | chromosome_16 |
| Location: | 3520681 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g677382 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AACCCCCGCCCTCCGGACCAACACCTGAAA |
| Internal bar code: | TAGGCCACGTGAGAGCTATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 807 |
| LEAP-Seq percent confirming: | 99.8146 |
| LEAP-Seq n confirming: | 8612 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTTACGGACTTCAGCC |
| Suggested primer 2: | GTGGTATCTGCTTCCCGTGT |