| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.118558 |
| Chromosome: | chromosome 12 |
| Location: | 468171 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCCTCGCCCCCCTCCCAGCCCTTGCGA |
| Internal bar code: | CGGCGATGAGACAAGGTCGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 72.4596 |
| LEAP-Seq n confirming: | 12992 |
| LEAP-Seq n nonconfirming: | 4938 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAAACCAAACCAAACTG |
| Suggested primer 2: | ACCCTGACACACAGGCCTAC |