Insertion junction: LMJ.RY0402.118569_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.118569
Chromosome:chromosome 17
Location:4129746
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g729650 MPA15 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR35769:SF2 - CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGCTGCTGAAGTTCTTGCCGCCCTGGTG
Internal bar code:ACCTGGTGGTCGCCGTGCTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:751
LEAP-Seq percent confirming:98.7578
LEAP-Seq n confirming:954
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTAGCTGCAGGGGTCTTC
Suggested primer 2:AACACACTCCCTCCCTCCTT