| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.118636 |
| Chromosome: | chromosome 1 |
| Location: | 4627498 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031800 | (1 of 1) PTHR23083//PTHR23083:SF433 - TETRATRICOPEPTIDE REPEAT PROTEIN, TPR // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCCTGACTCAGCCAGCATAACCCACTG |
| Internal bar code: | CTCGCGTGGTGCGCAAATGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 99.6283 |
| LEAP-Seq n confirming: | 15544 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCGTGTGTGTGAGAGAG |
| Suggested primer 2: | ATTGTTCATTGCTCTCGGCT |