| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.118666 |
| Chromosome: | chromosome_1 |
| Location: | 6117222 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g043650 | DLU2,MOT37 | Outer arm dynein light chain, ODA8 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCGAATGCTGCAGGTGCTGTGGGTGTCG |
| Internal bar code: | CGGCCGAACCGGAGCAGAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 73 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCACCAGTAGGTTCATCT |
| Suggested primer 2: | AGAACAAGGCTCGTGCTCAT |