| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.118669 |
| Chromosome: | chromosome 12 |
| Location: | 2685175 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503950 | (1 of 10) PTHR10174 - RETINALDEHYDE BINDING PROTEIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGCGCTCCTGCGGCATTAGTAATGAA |
| Internal bar code: | GAGATGGCCGGGGTACTCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 617 |
| LEAP-Seq percent confirming: | 99.636 |
| LEAP-Seq n confirming: | 1916 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGGAACGTTTCTCAAAA |
| Suggested primer 2: | AGAGTTGGGATGGGTGTCAG |