| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.118676 |
| Chromosome: | chromosome 14 |
| Location: | 265930 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609450 | PWR4 | (1 of 1) IPR000104//IPR006502 - Antifreeze protein, type I // Protein of unknown function PDDEXK-like; PWR motif protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGACAGCGCAAATTATCTTCATTTTAC |
| Internal bar code: | ATTCATAGAGCACGCATATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1024 |
| LEAP-Seq percent confirming: | 98.5995 |
| LEAP-Seq n confirming: | 9645 |
| LEAP-Seq n nonconfirming: | 137 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTAGAGCGACAGCGAGTT |
| Suggested primer 2: | CCCTTACAAGGTGCCCTACA |