| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.118680 |
| Chromosome: | chromosome 3 |
| Location: | 2536122 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160150 | (1 of 35) IPR016135 - Ubiquitin-conjugating enzyme/RWD-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTTGTGAGCTCCAGGTAGCTCCAGGTG |
| Internal bar code: | CTATGGCCGGAGGGCGCGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 99.2567 |
| LEAP-Seq n confirming: | 3472 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCAGCCGTTATCTCAAG |
| Suggested primer 2: | ATTCATTCACCCAACCCAAA |