| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.118745 |
| Chromosome: | chromosome 16 |
| Location: | 3146973 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAACACATCAAGCGCCTTCGGAACCTGA |
| Internal bar code: | TGCACTAGGTCTATAATCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.6485 |
| LEAP-Seq n confirming: | 567 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCACAGTCTCAGGCGTC |
| Suggested primer 2: | CAGAACCCTGTCCACGATTT |