Insertion junction: LMJ.RY0402.118751_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.118751
Chromosome:chromosome 1
Location:3136418
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g019550 ankryin repeat containing protein; (1 of 2) PTHR24128:SF17 - E3 UBIQUITIN-PROTEIN LIGASE XBAT32 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAGAGACGGGGCCGGGCTCAGCGCCGCG
Internal bar code:TCGTTGGGGTCCTCGTGTGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:647
LEAP-Seq percent confirming:98.3744
LEAP-Seq n confirming:2118
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTGTTGCTGATGTTGCT
Suggested primer 2:CTTCAGAGCTCGGGTGACTC