| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.118764 |
| Chromosome: | chromosome 17 |
| Location: | 746661 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g701250 | FLA12,PF8,CCDC39,FAP59 | Flagellar Associated Protein 59; (1 of 1) PTHR18962//PTHR18962:SF0 - FAMILY NOT NAMED // COILED-COIL DOMAIN-CONTAINING PROTEIN 39 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTCGCTGGTCACGTTTGTTGCGGTCAT |
| Internal bar code: | TGGGTTCACCCGCACTCGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 99.4939 |
| LEAP-Seq n confirming: | 26739 |
| LEAP-Seq n nonconfirming: | 136 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCCTCAATCACAAACCTC |
| Suggested primer 2: | TATCGGGAAGCTTGTGCTTT |