Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.118772 |
Chromosome: | chromosome_17 |
Location: | 3097625 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g721000 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTGAACGTGCCGCCCAGCGCGTACGTCAG |
Internal bar code: | AAAAGGGTGGCCCGACGGTCAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 466 |
LEAP-Seq percent confirming: | 99.6606 |
LEAP-Seq n confirming: | 881 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAACTGACGGTTAGGGCAC |
Suggested primer 2: | TGGTGTACCTGGTGAGTGGA |