| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.118799 |
| Chromosome: | chromosome 6 |
| Location: | 3567244 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278118 | 5'UTR_intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTAATGCAGTAAGAAATGGTACTTGTCG |
| Internal bar code: | GGTACCAGTAGGTATCTCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1166 |
| LEAP-Seq percent confirming: | 98.9691 |
| LEAP-Seq n confirming: | 288 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCACAACAGGTGAGCA |
| Suggested primer 2: | CAGGTAGGTAGCTTGGCGAG |