| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.118810 |
| Chromosome: | chromosome 8 |
| Location: | 2260795 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGCGGAAACGTAAGGAACGAAGGCAG |
| Internal bar code: | GATAGTCCAAGAACGGTGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 75.0978 |
| LEAP-Seq n confirming: | 1535 |
| LEAP-Seq n nonconfirming: | 509 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCTCCGAAGCAGTGTCT |
| Suggested primer 2: | AGTGACACCAGCAGCATCAG |