| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.118815 |
| Chromosome: | chromosome 10 |
| Location: | 5499756 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGTGTACCTTCCGGTTGCTCCCACATC |
| Internal bar code: | ATATAATTCCGTTCCCGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1008 |
| LEAP-Seq percent confirming: | 96.2373 |
| LEAP-Seq n confirming: | 14195 |
| LEAP-Seq n nonconfirming: | 555 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTAATGATCATGGCTGT |
| Suggested primer 2: | CTTTAGCAGCACGTCTTCCC |